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Proceedings Paper

Gold nanorods for cell imaging with confocal reflectance microscopy and two-photon fluorescence microscopy
Author(s): Ji-Yao Chen; Pei-Nan Wang
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Paper Abstract

Gold nanorods have unique optical properties as their two photon absorption cross sections are very high and their spectral positions of extinction bands can be controlled by their aspect ratio only, so that gold nanorods have been considered as agents for cell imaging. Two-photon photoluminescence imaging could be used to detect the cellular gold nanorods with the high power femto-second (fs) infrared laser, but may cause the photothermal effect melting the rods. The 3-D distribution of gold nanorods in living cells also can be measured by confocal reflectance microscopy with a very low laser power, and thus the cell damaging can be avoided. In this work, these two methods were comparatively studied in living rat basophilic leukemia (RBL-2H3) cells.

Paper Details

Date Published: 24 February 2010
PDF: 6 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680A (24 February 2010); doi: 10.1117/12.838703
Show Author Affiliations
Ji-Yao Chen, Fudan Univ. (China)
Pei-Nan Wang, Fudan Univ. (China)

Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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