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Proceedings Paper

Spatio-temporal control in multiphoton fluorescence laser-scanning microscopy
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Paper Abstract

The broad spectral window of an ultra-short laser pulse and the broad overlapping multiphoton absorption spectra of common fluorophores restrict selective excitation of one fluorophore in presence of others during multiphoton fluorescence microscopy. Also spatial resolution, limited by the fundamental diffraction limit, is governed by the beam profile. Here we show our recent work on selective fluorescence suppression using a femtosecond pulse-pair excitation which is equivalent to amplitude shaping using a pulse shaper. In addition, prospects of laser beam shaping in imaging are also briefly discussed.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756929 (26 February 2010); doi: 10.1117/12.838287
Show Author Affiliations
Arijit Kumar De, Indian Institute of Technology Kanpur (India)
Debjit Roy, Indian Institute of Technology Kanpur (India)
Debabrata Goswami, Indian Institute of Technology Kanpur (India)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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