We developed a multifocus excitation coherent anti-Stokes Raman scattering microscope using a microlens array scanner for realtime molecular imaging. Two picoseond mode-locked lasers tightly synchronized were splited to a few tens of foci with the microlens array, the foci excited the sample parallely and the generated CARS from each spot was detected with an image sensor at once. By the multifocus excitation, exposure time was prolonged proportionally to the number of the foci because of parallel excitation and detection. The video-rate (frame rate of 30 fps) imaging of polystyrene beads in water was demonstrated, and the Brownian motion of beads were clearly obtained. The three-dimensional reconstructed imaging of living HeLa cells (frame rate of 5 fps, 85 images) was also demonstrated.

Date Published: 2 December 2009
PDF: 8 pages
Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070H (2 December 2009); doi: 10.1117/12.838084

Published in SPIE Proceedings Vol. 7507:
2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging
Xiaocong Yuan; Yinmei Li; Arthur Chiou; Min Gu; Dennis Matthews; Colin Sheppard, Editor(s)