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Proceedings Paper

Light scattering characterization of single biological cells in a microfluidic cytometer
Author(s): Xuantao Su; Sean E. Kirkwood; Hilal Gul; Kirat Singh; Md. Z. Islam; Anna Janowska-Wieczorek; Wojciech Rozmus; Ying Y. Tsui
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Paper Abstract

The characterization of single biological cells in a microfluidic flow by using a 2D light scattering microfluidic cytometric technique is described. Laser light is coupled into a microfluidic cytometer via an optical fiber to illuminate a single scatterer in a fluidic flow. The 2D light scattering patterns are obtained by using a charge-coupled device (CCD) detector. The system is tested by using standard polystyrene beads of 4 μm and 9.6 μm in diameter, and the bead experimental results agree well with 1D Mie theory simulation results. Experiments on yeast cells are performed using the microfluidic cytometer. Cell results are studied by finite-difference time-domain (FDTD) method, which can simulate light scattering from non-homogeneous cells. For example, a complex biological cell model with inner mitochondrial distribution is studied by FDTD in this paper. Considering the yeast cell size variations, the yeast cell 2D scatter patterns agree well with the FDTD 2D simulation patterns. The system is capable of obtaining 2D side scatter patterns from a single biological cell which may contain rich information on the biological cell inner structures. The integration of light scattering, microfluidics and fiber optics described here may ultimately allow the development of a lab-on-chip cytometer for label-free detection of diseases at a single cell level.

Paper Details

Date Published: 3 August 2009
PDF: 8 pages
Proc. SPIE 7386, Photonics North 2009, 738602 (3 August 2009);
Show Author Affiliations
Xuantao Su, Univ. of Alberta (Canada)
Sean E. Kirkwood, Univ. of Alberta (Canada)
Hilal Gul, Univ. of Alberta (Canada)
Canadian Blood Services (Canada)
Kirat Singh, Univ. of Alberta (Canada)
Md. Z. Islam, Univ. of Alberta (Canada)
Anna Janowska-Wieczorek, Univ. of Alberta (Canada)
Canadian Blood Services (Canada)
Wojciech Rozmus, Univ. of Alberta (Canada)
Ying Y. Tsui, Univ. of Alberta (Canada)

Published in SPIE Proceedings Vol. 7386:
Photonics North 2009
Réal Vallée, Editor(s)

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