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Proceedings Paper

Design and optimization for microscope autofocus systems based on the analysis of video signals
Author(s): Peng Ge; Bangfu Mao; Huajun Feng; Zhihai Xu; Qi Li
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Paper Abstract

An autofocus system applied to microscope has been designed, which is based on the analysis of analog video signals' processing. A multi-feedback band-pass active filter retains high frequency components of analog video signals from CCD, then high frequency components that represent image details information are amplified and performed integral operation, which is defined as focusing measure. After A/D transformation a MCU controls the focusing procedure and drives a stepping motor to find focused-position. The selection of bandwidths of filter, selection of focusing windows, step-size in search and hill climbing algorithms are discussed and optimized to improve focus accuracy and shorten focus time.

Paper Details

Date Published: 30 October 2009
PDF: 7 pages
Proc. SPIE 7495, MIPPR 2009: Automatic Target Recognition and Image Analysis, 74952U (30 October 2009); doi: 10.1117/12.832537
Show Author Affiliations
Peng Ge, Zhejiang Univ. (China)
Bangfu Mao, Zhejiang Univ. (China)
Huajun Feng, Zhejiang Univ. (China)
Zhihai Xu, Zhejiang Univ. (China)
Qi Li, Zhejiang Univ. (China)

Published in SPIE Proceedings Vol. 7495:
MIPPR 2009: Automatic Target Recognition and Image Analysis
Tianxu Zhang; Bruce Hirsch; Zhiguo Cao; Hanqing Lu, Editor(s)

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