Extended focus optical coherence microscope (xfOCM) circumvents the compromise between lateral resolution and depth of field by us of a Bessel-like illumination beam. The high sensitivity and parallel depth profiling of Fourier domain optical coherence tomography are preserved, and combined with high isotropic resolution of 1.5 - 2 μm. To comply with the requirements for in vivo measurements, beam scanning had to be implemented. We then performed measurements, first of excised pancreas, validated by standard immunohistochemistry, to investigate the structures that can be observed. For a quantitative analysis, a semi-automatic islet detection algorithm evaluated the islet size, position, contrast and homogeneity. The influence of streptozotocin on the signature of the islets was investigated in a next step. Finally, xfOCM was applied to make measurements of the murine pancreas in situ and in vivo, visualizing pancreatic lobules, ducts, blood vessels and individual islets of Langerhans.

Date Published: 13 July 2009
PDF: 12 pages
Proc. SPIE 7372, Optical Coherence Tomography and Coherence Techniques IV, 73720E (13 July 2009); doi: 10.1117/12.831864

Published in SPIE Proceedings Vol. 7372:
Optical Coherence Tomography and Coherence Techniques IV
Peter E. Andersen; Brett E. Bouma, Editor(s)