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Proceedings Paper

Improving FRET detection in living cells
Author(s): Ching-Wei Chang; Mei Wu; Sofia D. Merajver M.D.; Mary-Ann Mycek
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Paper Abstract

Unambiguous FRET detection in living cells is often challenging. Here we describe how the advantages of fluorescence lifetime sensing with FLIM, fluorophore selection, and critical environmental controls provide better FRET detection.

Paper Details

Date Published: 1 July 2009
PDF: 7 pages
Proc. SPIE 7370, Molecular Imaging II, 737007 (1 July 2009); doi: 10.1117/12.831718
Show Author Affiliations
Ching-Wei Chang, Univ. of Michigan, Ann Arbor (United States)
Mei Wu, Univ. of Michigan, Ann Arbor (United States)
Sofia D. Merajver M.D., Univ. of Michigan, Ann Arbor (United States)
Mary-Ann Mycek, Univ. of Michigan, Ann Arbor (United States)


Published in SPIE Proceedings Vol. 7370:
Molecular Imaging II
Kai Licha; Charles P. Lin, Editor(s)

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