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Proceedings Paper

Correction of Raman signals for tissue optical properties
Author(s): Carina Reble; Ingo Gersonde; Jürgen Helfmann; Stefan Andree; Gerd Illing
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Paper Abstract

Raman signals of biological tissues are distorted by the influence of tissue absorption and scattering properties, which significantly challenges signal quantification. We investigated the influence of the tissue optical properties on the resonance Raman signal of β-carotene with tissue phantom measurements and Monte Carlo simulations. Both methods show that the Raman signal drops roughly proportional to 1/μa for absorption coefficients (μa) in the range of 0.1-4mm-1. The influence of the reduced scattering coefficient in the range of 0.1-8mm-1 is less strong, but not negligible. We introduce combined Raman and spatially resolved reflectance measurements to measure both Raman signals and tissue optical properties. The optical properties were deduced from the spatially resolved reflectance measurements by a Monte Carlo-based lookup table (LUT). For the signal correction we applied correction functions derived from the Monte Carlo simulations. We corrected in vivo resonance Raman measurements of carotenoids in caucasian skin (n=9) with respect to the mean optical properties of the group. The magnitude of the average correction effect was found to be 18±11% on the minimal pigmented palm.

Paper Details

Date Published: 7 July 2009
PDF: 11 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680C (7 July 2009); doi: 10.1117/12.831595
Show Author Affiliations
Carina Reble, Laser und Medizin Technologie Gmbh, Berlin (Germany)
Technical Univ. Berlin (Germany)
Ingo Gersonde, Laser und Medizin Technologie Gmbh, Berlin (Germany)
Jürgen Helfmann, Laser und Medizin Technologie Gmbh, Berlin (Germany)
Stefan Andree, Laser und Medizin Technologie Gmbh, Berlin (Germany)
Technical Univ. Berlin (Germany)
Gerd Illing, Laser und Medizin Technologie Gmbh, Berlin (Germany)

Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg M.D., Editor(s)

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