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Proceedings Paper

Efficiency of fluorescence coupling into planar waveguides
Author(s): R. Bäumner; K. Bodensiek; A. Selle; T. Fricke-Begemann; J. Ihlemann; G. Marowsky
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Paper Abstract

Using planar waveguides as a platform for optical biosensors allows an efficient and selective fluorescence excitation in close proximity to the waveguide surface. Usually, the fluorescence light that is emitted in the space above the sensor chip is collected and analyzed by suitable free space optics and a detector. Due to the vicinity of the fluorescent molecules to the interface of the waveguide layer, a substantial part of the fluorescence light is coupled back into and collected by the waveguide. The coupling efficiency depends on position, environment and orientation of the molecules. The utilization of this signal for fluorescence detection and analysis can allow a significant simplification of the optical instrumentation. We present a fundamental investigation of the fluorescence collection efficiency into the waveguide by theoretical and experimental means.

Paper Details

Date Published: 7 July 2009
PDF: 7 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 736828 (7 July 2009); doi: 10.1117/12.831579
Show Author Affiliations
R. Bäumner, Laser-Lab. Göttingen e.V. (Germany)
K. Bodensiek, Laser-Lab. Göttingen e.V. (Germany)
A. Selle, Laser-Lab. Göttingen e.V. (Germany)
T. Fricke-Begemann, Laser-Lab. Göttingen e.V. (Germany)
J. Ihlemann, Laser-Lab. Göttingen e.V. (Germany)
G. Marowsky, Laser-Lab. Göttingen e.V. (Germany)

Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg M.D., Editor(s)

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