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Proceedings Paper

Quasi white light multiphoton imaging
Author(s): C. de Mauro; D. Alfieri; M. Arrigoni; D. Armstrong; F. S. Pavone
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Paper Abstract

We describe the realization and characterization of a broadband, high power density and fully spectrally controllable source, suitable for multiphoton imaging of biological samples. We used a photonic crystal fiber (PCF) with selected dispersive and non-linear properties, in order to generate, when pumped with <140 femtosecond pulses delivered by a tunable Ti:Sa laser (Chameleon Ultra II by Coherent Inc.), a smooth continuum in the 700nm-950nm region, with average power density grater than 2mW/nm. Time distribution of the generated spectrum has been measured with autocorrelation technique. Axial and lateral resolution obtained with a scanning multiphoton system has been determined to be near the theoretical limit. The possibility of two-photon excitation of different dyes in the same sample and high image resolution are demonstrated at tens of microns in depth. Future developments and different applications are also discussed.

Paper Details

Date Published: 1 July 2009
PDF: 7 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670U (1 July 2009); doi: 10.1117/12.831549
Show Author Affiliations
C. de Mauro, Light4Tech Firenze S.r.l. (Italy)
D. Alfieri, Light4Tech Firenze S.r.l. (Italy)
M. Arrigoni, Coherent, Inc. (United States)
D. Armstrong, Coherent, Inc. (United States)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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