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Dose limited fluorescence microscopy of living cells
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Paper Abstract

Light dose plays an important role for maintaining viability in optical microscopy of living cells. Therefore, a colony forming assay was established, and non-phototoxic light doses were determined for glioblastoma cells. These doses ranged from a few 1 J/cm2 or even less for cells incubated with fluorescence markers or photosensitizers up to about 100 J/cm2 for non-incubated cells. Microscopic methods were adapted to those light doses, and often wide field methods appeared to be more appropriate than laser scanning methods.

Paper Details

Date Published: 1 July 2009
PDF: 6 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670E (1 July 2009); doi: 10.1117/12.831547
Show Author Affiliations
Herbert Schneckenburger, Hochschule Aalen (Germany)
Univ. Ulm (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Petra Weber, Hochschule Aalen (Germany)
Sarah Schickinger, Hochschule Aalen (Germany)
Thomas Bruns, Hochschule Aalen (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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