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Proceedings Paper

Study of 3D cell morphology and effect on light scattering distribution
Author(s): Andrew E. Ekpenyong; Junhua Ding; Li V. Yang; Nancy R. Leffler; Jun Q. Lu; R. Scott Brock; Xin-Hua Hu
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Paper Abstract

We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

Paper Details

Date Published: 1 July 2009
PDF: 7 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73671J (1 July 2009); doi: 10.1117/12.831510
Show Author Affiliations
Andrew E. Ekpenyong, East Carolina Univ. (United States)
Junhua Ding, East Carolina Univ. (United States)
Li V. Yang, East Carolina Univ. (United States)
Nancy R. Leffler, East Carolina Univ. (United States)
Jun Q. Lu, East Carolina Univ. (United States)
R. Scott Brock, Virginia Commonwealth Univ. (United States)
Xin-Hua Hu, East Carolina Univ. (United States)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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