
Proceedings Paper
A comparison between coherent and spontaneous Raman scattering for biological imagingFormat | Member Price | Non-Member Price |
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Paper Abstract
We compare imaging using coherent and spontaneous Raman scattering under biological imaging conditions. We
perform spectral domain imaging of polystyrene beads and find comparable signal levels for both methods at excitation
powers and concentrations most relevant for biological samples. The critical power at which the two methods provide
equivalent signal levels is found to be ~1.3 mW in 10 M polystyrene beads and ~7 mW in 13 M 2-propanol. The low
sample concentrations and low excitation power necessary for most biological imaging applications reduce the relative
advantages offered by coherent Raman methods.
Paper Details
Date Published: 1 July 2009
PDF: 5 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670V (1 July 2009); doi: 10.1117/12.831497
Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)
PDF: 5 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670V (1 July 2009); doi: 10.1117/12.831497
Show Author Affiliations
Brandon R. Bachler, Univ. of Michigan (United States)
Meng Cui, Univ. of Michigan (United States)
Meng Cui, Univ. of Michigan (United States)
Sarah R. Nichols, Univ. of Michigan (United States)
Jennifer P. Ogilvie, Univ. of Michigan (United States)
Jennifer P. Ogilvie, Univ. of Michigan (United States)
Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)
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