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Proceedings Paper

Time- and spectral-resolved multiphoton imaging of fresh bladder biopsies
Author(s): Riccardo Cicchi; Alfonso Crisci; Gabriella Nesi; Alessandro Cosci; Saverio Giancane; Marco Carini; Francesco S. Pavone
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Paper Abstract

Human tissues intrinsically contains many fluorophores, as such NADH, elastin, collagen, and flavins, that can be excited and imaged using multiphoton microscopy, up to 150 microns depth. In this work we used combined two photon intrinsic fluorescence (TPE), second harmonic generation microscopy (SHG), fluorescence lifetime imaging microscopy (FLIM), and multispectral two photon emission detection (MTPE) to investigate different kinds of human ex-vivo fresh biopsies of bladder. Morphological and spectroscopic analyses allowed to characterize both healthy and pathological tissue samples in a good agreement with common routine histology. In particular, we examined tissue samples from bladder normal mucosa, and bladder carcinoma in-situ (CIS), finding both morphological and spectroscopic differences. From the morphological point of view, cancer cells appeared more elongated with respect to corresponding normal cells; they also exhibited a different nucleus to cytoplasm ratio. From the spectroscopic point of view, we found differences between the two tissue types in both spectral emission and fluorescence lifetime distribution. Even if further analysis, as well as a more significant statistics on a large number of samples would be helpful to discriminate between low and high grade cancer, our method is a promising tool to be used as diagnostic confirmation of histological results, as well as a diagnostic tool in a multiphoton endoscope or cystoscope to be used in in-vivo imaging applications.

Paper Details

Date Published: 1 July 2009
PDF: 9 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670M (1 July 2009); doi: 10.1117/12.831486
Show Author Affiliations
Riccardo Cicchi, European Lab. for Non-linear Spectroscopy-L.E.N.S. and Univ. degli Studi di Firenze (Italy)
Alfonso Crisci, Univ. of Florence Medical School (Italy)
Gabriella Nesi, Univ. of Florence Medical School (Italy)
Alessandro Cosci, European Lab. for Non-linear Spectroscopy-L.E.N.S. and Univ. degli Studi di Firenze (Italy)
Saverio Giancane, Univ. of Florence Medical School (Italy)
Marco Carini, Univ. of Florence Medical School (Italy)
Francesco S. Pavone, European Lab. for Non-linear Spectroscopy-L.E.N.S. and Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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