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Proceedings Paper

Rapid test for the detection of hazardous microbiological material
Author(s): Mario Mordmueller; Christian Bohling; Andreas John; Wolfgang Schade
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Paper Abstract

After attacks with anthrax pathogens have been committed since 2001 all over the world the fast detection and determination of biological samples has attracted interest. A very promising method for a rapid test is Laser Induced Breakdown Spectroscopy (LIBS). LIBS is an optical method which uses time-resolved or time-integrated spectral analysis of optical plasma emission after pulsed laser excitation. Even though LIBS is well established for the determination of metals and other inorganic materials the analysis of microbiological organisms is difficult due to their very similar stoichiometric composition. To analyze similar LIBS-spectra computer assisted chemometrics is a very useful approach. In this paper we report on first results of developing a compact and fully automated rapid test for the detection of hazardous microbiological material. Experiments have been carried out with two setups: A bulky one which is composed of standard laboratory components and a compact one consisting of miniaturized industrial components. Both setups work at an excitation wavelength of λ=1064nm (Nd:YAG). Data analysis is done by Principal Component Analysis (PCA) with an adjacent neural network for fully automated sample identification.

Paper Details

Date Published: 17 September 2009
PDF: 10 pages
Proc. SPIE 7484, Optically Based Biological and Chemical Detection for Defence V, 74840F (17 September 2009); doi: 10.1117/12.830200
Show Author Affiliations
Mario Mordmueller, Clausthal Univ. of Technology (Germany)
Christian Bohling, SECOPTA GmbH (Germany)
Andreas John, SECOPTA GmbH (Germany)
Wolfgang Schade, Clausthal Univ. of Technology (Germany)
Fraunhofer Institute for Telecommunications (Germany)

Published in SPIE Proceedings Vol. 7484:
Optically Based Biological and Chemical Detection for Defence V
John C. Carrano; Charles J. Collins, Editor(s)

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