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Proceedings Paper

Boundary-condition dependent elasticity of short double-stranded DNA molecules
Author(s): Yih-Fan Chen; K. Raghunathan; David Wilson; Jens-Christian Meiners
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Paper Abstract

We have measured the entropic elasticity of ds-DNA molecules ranging from 247 to 1298 base pairs in length, using axial optical force-clamp tweezers. We show that entropic end effects and excluded-volume forces become significant for such short molecules. In this geometry, the effective persistence length of the shortest molecules decreases by a factor of two compared to the established value for long molecules, and excluded-volume forces extend the molecules to about one third of their nominal contour lengths in the absence of any external forces. We interpret these results in the framework of a modified wormlike chain model.

Paper Details

Date Published: 20 August 2009
PDF: 9 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740003 (20 August 2009); doi: 10.1117/12.827671
Show Author Affiliations
Yih-Fan Chen, Univ. of Michigan (United States)
K. Raghunathan, Univ. of Michigan (United States)
David Wilson, Univ. of Michigan (United States)
Jens-Christian Meiners, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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