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Proceedings Paper

SLM-based phase-contrast filtering for single and multiple image acquisition
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Paper Abstract

We report on the implementation of different phase contrast methods using an SLM in a microscope. The ease of generating complex phase filters with the SLM opens the possibility to realize standard filters adapted to the specimen and the possibility to develop new phase contrasting methods. Due to the real-time addressing we can obtain a number of different images from each specimen recorded nearly simultaneously with the same microscope objective. We demonstrate how to realize different versions of differential interference contrast imaging, Zernike-type imaging and dark-field imaging and the combination of the different images by simple post processing. Experimental results for biological as well as technical specimens are presented.

Paper Details

Date Published: 24 August 2009
PDF: 12 pages
Proc. SPIE 7442, Optics and Photonics for Information Processing III, 74420E (24 August 2009); doi: 10.1117/12.825945
Show Author Affiliations
Michael Warber, Univ. Stuttgart (Germany)
Susanne Zwick, Univ. Stuttgart (Germany)
Malte Hasler, Univ. Stuttgart (Germany)
Tobias Haist, Univ. Stuttgart (Germany)
Wolfgang Osten, Univ. Stuttgart (Germany)

Published in SPIE Proceedings Vol. 7442:
Optics and Photonics for Information Processing III
Khan M. Iftekharuddin; Abdul Ahad Sami Awwal, Editor(s)

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