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Proceedings Paper

Nonlinear optical properties of photoswitchable fluorescent proteins
Author(s): Inge Asselberghs; Cristina Flors; Evelien De Meulenaere; Benoît Champagne; Jos Vanderleyden; Koen Clays
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Paper Abstract

and enhanced GFP (eGFP), enhanced YFP (eYFP) and DsRed have been studied at both the theoretical and experimental levels. In the case of Dronpa, both approaches are consistent in showing the rather counterintuitive result of a larger second-order nonlinear polarizability (or first hyperpolarizability) for the protonated state, which has a higher transition energy, than for the deprotonated, fluorescent state with its absorption at lower energy. Moreover, the hyperpolarizability value for the protonated form of Dronpa is among the highest reported for proteins. In addition to the pH dependence, we have found wavelength dependence in the values. These properties are essential for the practical use of Dronpa or other GFP-like fluorescent proteins as second-order nonlinear fluorophores for symmetry-sensitive nonlinear microscopy imaging and as nonlinear optical sensors for electrophysiological processes. An accurate value of the first hyperpolarizability is also essential for any qualitative analysis of the nonlinear images.

Paper Details

Date Published: 20 August 2009
PDF: 7 pages
Proc. SPIE 7403, Nanobiosystems: Processing, Characterization, and Applications II, 74030P (20 August 2009); doi: 10.1117/12.824552
Show Author Affiliations
Inge Asselberghs, Katholieke Univ. Leuven (Belgium)
Cristina Flors, Katholieke Univ. Leuven (Belgium)
Evelien De Meulenaere, Katholieke Univ. Leuven (Belgium)
Univ. of Leuven (Belgium)
Benoît Champagne, Lab de Chimie Théorique Appliquée, Univ. Notre-Dame de la Paix (Belgium)
Jos Vanderleyden, Univ. of Leuven (Belgium)
Koen Clays, Katholieke Univ. Leuven (Belgium)

Published in SPIE Proceedings Vol. 7403:
Nanobiosystems: Processing, Characterization, and Applications II
Norihisa Kobayashi; Fahima Ouchen; Ileana Rau, Editor(s)

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