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Proceedings Paper

Fiber optic SERS-based plasmonics nanobiosensing in single living cells
Author(s): Jonathan P. Scaffidi; Molly K. Gregas; Victoria Seewaldt; Tuan Vo-Dinh
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Paper Abstract

We describe the development of small molecule-sensitive plasmonics-active fiber-optic nanoprobes suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The practical utility of SERS-based fiber-optic nanoprobes is illustrated by measurements of intracellular pH in HMEC- 15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor biologically-relevant small molecules at the single cell level.

Paper Details

Date Published: 30 April 2009
PDF: 8 pages
Proc. SPIE 7312, Advanced Environmental, Chemical, and Biological Sensing Technologies VI, 731205 (30 April 2009); doi: 10.1117/12.824453
Show Author Affiliations
Jonathan P. Scaffidi, Duke Univ. (United States)
Molly K. Gregas, Duke Univ. (United States)
Victoria Seewaldt, Duke Univ. (United States)
Duke Univ. School of Medicine (United States)
Tuan Vo-Dinh, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 7312:
Advanced Environmental, Chemical, and Biological Sensing Technologies VI
Tuan Vo-Dinh; Robert A. Lieberman; Günter Gauglitz, Editor(s)

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