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Proceedings Paper

Cisplatin-induced Casepase-3 activation in different tumor cells
Author(s): Hua Shi; Xiao Li; Ting Su; Yu-Hai Zhang
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Paper Abstract

Apoptosis plays an essential role in normal organism development which is one of the main types of programmed cell death to help tissues maintain homeostasis. Defective apoptosis can result in cell accumulation and therefore effects on tumor pathogenesis, progression and therapy resistance. A family of proteins, known as caspases, is typically activated in the early stages of apoptosis. Therefore, studying the kinetics of activation of caspases induced by antitumor drugs can contribute to antitumor drug discovery and explanation of the molecular mechanisms. This paper detected the Caspase-3 activity induced by cisplatin in human adenoid cystic carcinoma cell line (ACC-M), human hepatocellular liver carcinoma cell line (HepG2) and human epithelial carcinoma cell line (Hela) with stably expressing ECFP-DEVDDsRed (CD3) probe, a fluorescent probe consisting of Enhanced Cyan Fluorescent Protein (ECFP), red fluorescent protein (DsRed) and a linker with a recognition site of Caspase-3, by using the capillary electrophoresis (CE) and fluorescence resonance energy transfer (FRET) imaging system. Under the same concentration of cisplatin, ACC-M cells responded the most rapidly, and then HepG2 cells and Hela cells, respectively, in the early 30 hours. Later, HepG2 cells represented acceleration in the Caspase-3 activation speed and reached full activation the earliest comparing to other two cell types. The results demonstrated that ACC-M cell is more sensitive than the other two cell types under the treatment of cisplatin.

Paper Details

Date Published: 6 March 2009
PDF: 7 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801S (6 March 2009); doi: 10.1117/12.824107
Show Author Affiliations
Hua Shi, Huazhong Univ. of Science and Technology (China)
Xiao Li, Huazhong Univ. of Science and Technology (China)
Ting Su, Huazhong Univ. of Science and Technology (China)
Yu-Hai Zhang, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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