
Proceedings Paper
Novel PDD-PDT system based on spectrophotometric real-time fluorescence monitoring and MALDI-TOF-MS analysis of tumorsFormat | Member Price | Non-Member Price |
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Paper Abstract
In the PDT practice for tumor patients, the dose and irradiation time for the treatment are chosen by experience and not
by real need. To establish advanced PDD-PDT model system for patients, we developed a method for monitoring the
cell-death based on a spectrophotometric real-time change in fluorescence in HeLa-tumors during Photofrin®-PDT and
ALA-PDT. Here, we describe the results of application of the new PDD-PDT system to human tumors. The fluorescence
spectra obtained from human tumors were analyzed by the differential spectral analysis. The mass-spectral changes of
tumor tissues during PDD-PDT were also examined by MALDI-TOF-MS/MS. The first author's seborrheic keratosis was
monitored with this system during the PDD-PDT with a topically applied ALA-ointment. The changes in fluorescence
spectrum were successfully detected, and the tumor regressed completely within 5 months. The differential spectral
analysis of PDD-PDT-fluorescence monitoring spectra of tumors and isolated mitochondria showed a marked decrease of
three peaks in the red region indicative of the PDD (600 - 720 nm), and a transient rise followed by a decline of peaks in
the green region indicative of the PDT (450 - 580 nm). The MALDI-TOF-MS analysis of PDD-PDT HeLa-tumors
showed a consumption of Photofrin-deuteroporphyrin and ALA-PpIX, and decreases in protein mass in the range of
4,000 - 16,000 Da, m/z 4929, 8564, 10089, 15000, and an increase in m/z 7002 in a Photofrin® PDD-PDT monitoring
tumor.
Paper Details
Date Published: 13 July 2009
PDF: 6 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73801I (13 July 2009); doi: 10.1117/12.823247
Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)
PDF: 6 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73801I (13 July 2009); doi: 10.1117/12.823247
Show Author Affiliations
Takato O. Yoshida, Hamamatsu Univ. School of Medicine (Japan)
Eiji Kohno, Hamamatsu Univ. School of Medicine (Japan)
Marc Dodeller, Hamamatsu Univ. School of Medicine (Japan)
Eiji Kohno, Hamamatsu Univ. School of Medicine (Japan)
Marc Dodeller, Hamamatsu Univ. School of Medicine (Japan)
Takashi Sakurai, Hamamatsu Univ. School of Medicine (Japan)
Seiji Yamamoto, Hamamatsu Univ. School of Medicine (Japan)
Susumu Terakawa, Hamamatsu Univ. School of Medicine (Japan)
Seiji Yamamoto, Hamamatsu Univ. School of Medicine (Japan)
Susumu Terakawa, Hamamatsu Univ. School of Medicine (Japan)
Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)
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