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Proceedings Paper

Antimicrobial photodynamic therapy in a mouse model of Acinetobacter baumannii burn infection
Author(s): Tianhong Dai; George P. Tegos; Zongshun Lu; Timur Zhiyentayev; Liyi Huang; Michael J. Franklin; David G Baer; Michael R. Hamblin
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Paper Abstract

Multi-drug resistant Acinetobacter baumanii infections represent a growing problem, especially in traumatic wounds and burns suffered by military personnel injured in Middle Eastern conflicts. Effective treatment using traditional antibiotics can be extremely difficult and new antimicrobial approaches are being investigated. One of these antimicrobial alternatives could be the combination of non-toxic photosensitizers (PS) and visible light known as photodynamic therapy (PDT). We report on the establishment of a new mouse model of full thickness thermal burns infected with a bioluminescent derivative of a clinical Iraqi isolate of A. baumannii and its PDT treatment by topical application of a PS produced by covalent conjugation chlorin(e6) to polyethylenimine followed by illumination of the burn surface with red light. Application of 108 A. baumannii cells to the surface of 10-second burns made on the dorsal surface of shaved female BALB/c mice led to chronic infections that lasted on average 22 days characterized by a remarkably stable bacterial bioluminescence. PDT carried out on day 0 soon after applying bacteria gave over three logs of loss of bacterial luminescence in a light exposure dependent manner, while PDT carried out on day 1 and day 2 gave approximately a 1.7-log reduction. Application of PS dissolved in 10% or 20% DMSO without light gave only modest reduction in bacterial luminescence from mouse burns. Some bacterial regrowth in the treated burn was observed but was generally modest. It was also found that PDT did not lead to inhibition of wound healing. The data suggest that PDT may be an effective new treatment for multi-drug resistant localized A. baumannii infections.

Paper Details

Date Published: 13 July 2009
PDF: 9 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738037 (13 July 2009); doi: 10.1117/12.823065
Show Author Affiliations
Tianhong Dai, Wellman Ctr. for Photomedicine, MGH (United States)
Harvard Medical School (United States)
George P. Tegos, Wellman Ctr. for Photomedicine, MGH (United States)
Harvard Medical School (United States)
Zongshun Lu, Wellman Ctr. for Photomedicine, MGH (United States)
Tianjin Medical Univ. (China)
Timur Zhiyentayev, Wellman Ctr. for Photomedicine, MGH (United States)
M.V. Lomonosov Moscow State Univ. (Russian Federation)
Liyi Huang, Wellman Ctr. for Photomedicine, MGH (United States)
Harvard Medical School (United States)
Guangxi Medical Univ. (China)
Michael J. Franklin, Montana State Univ. (United States)
David G Baer, US Army Institute of Surgical Research (United States)
Michael R. Hamblin, Wellman Ctr. for Photomedicine, MGH (United States)
Harvard Medical School (United States)
Harvard-MIT Division of Health Sciences and Technology (United States)


Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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