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Proceedings Paper

Photodynamic inactivation of Gram (-) and Gram (+) microorganisms by cationic porphyrins and metalloporphyrins
Author(s): Grigor V. Gyulkhandanyan; Marina H. Paronyan; Anichka S. Hovsepyan; Robert K. Ghazaryan; Artak G. Tovmasyan; Aram G. Gyulkhandanyan; Anna G. Gyulkhandanyan; Gayane V. Amelyan
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Paper Abstract

Photodynamic inactivation (PDI) of microorganisms is successfully applied against Gram (+) microorganisms. However the majority of photosensitizers poorly affect on Gram (-) microorganisms. At present number of works have shown that cationic photosensitizers are able to induce photoinactivation both Gram (+) and Gram (-) microorganisms. The purpose of this work was definition of more effective new cationic pyridylporphyrins with various coordinated metals and functional groups for destruction of Gram (-) microorganisms. The efficiency of new cationic porphyrins and metalloporphyrins (9 compounds) was tested against Gram (-) microorganism E. coli (strain Κ-12). The testing results show high efficiency of metalloporphyrins, especially silver complexes, against E. coli microorganism under dark conditions. 50 % and 100 % cell growth inhibitory concentrations (IC50 and IC100 values, accordingly) of studied metallocomplexes are considerably lower in comparison with metal-free porphyrins. At the same time the Zncomplexes of porphyrins are more phototoxic than their metal-free analogues. Zn-metalloporphyrins with allyl and butyl functional groups were shown the highest efficiency against E. coli. The photodynamic action of cationic Zn -TBut4PyP metalloporphyrin against Gram(+) (St. aureus and St. epidermis) and Gram(-) (E.coli, strain K-12 and Salmonella sp.) microorganisms was investigated. It is revealed, that Gram (+) microorganisms were 3-5 times more susceptible to the compounds' phototoxic influence than Gram (-) microorganisms.

Paper Details

Date Published: 13 July 2009
PDF: 7 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73803I (13 July 2009); doi: 10.1117/12.823002
Show Author Affiliations
Grigor V. Gyulkhandanyan, Institute of Biotechnology (Armenia)
Marina H. Paronyan, Institute of Biotechnology (Armenia)
Anichka S. Hovsepyan, Institute of Biotechnology (Armenia)
Robert K. Ghazaryan, Yerevan State Medical Univ. (Armenia)
Artak G. Tovmasyan, Yerevan State Medical Univ. (Armenia)
Aram G. Gyulkhandanyan, Yerevan State Univ. (Armenia)
Anna G. Gyulkhandanyan, Yerevan State Univ. (Armenia)
Gayane V. Amelyan, Institute of Biotechnology (Armenia)

Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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