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Proceedings Paper

The new application of photosensitization reaction to atrial fibrillation treatment: mechanism and demonstration of non-thermal electrical conduction block with porcine heart
Author(s): Arisa Ito; Hiroki Matsuo; Tsukasa Suenari; Takuro Kajihara; Takehiro Kimura; Shunichiro Miyoshi; Satoshi Ogawa; Tsunenori Arai
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Paper Abstract

We have proposed non-thermal electrical conduction block for atrial fibrillation treatment by the photosensitization reaction, in which the interval time between the photosensitizer injection and irradiation is less than tenth of that in conventional way. To study the mechanism of photosensitization reaction-induced electrical conduction block, intracellular Ca2+ concentration change in rat myocardial cells was measured by fluorescent Ca2+ indicator Fluo-4 AM with confocal laser microscopy. Measured rapid increase in the fluorescence intensity and a change in cell morphology indicated that cell membrane damage; that is Ca2+ influx and eventually cell death caused by the photosensitization reaction. To demonstrate myocardial electrical conduction block induced by the photosensitization reaction, surgically exposed porcine heart under deep anesthesia was used. The myocardial tissue was paced with a stimulation electrode. The propagated electrical signals were measured by bipolar electrodes at two different positions. Thirty minutes after the injection of 5-10 mg/kg Porfimer sodium or Talaporfin sodium, the red laser light was irradiated to the tissue point by point crossing the measuring positions by the total energy density of less than 200 J/cm2. The electrical signal conduction between the measuring electrodes in the myocardial tissue was delayed by each irradiation procedure. The electrical conduction delay corresponded to the block line length was obtained. These results demonstrated the possibility of non-thermal electrical conduction block for atrial fibrillation treatment by the photosensitization reaction.

Paper Details

Date Published: 13 July 2009
PDF: 8 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738051 (13 July 2009); doi: 10.1117/12.822924
Show Author Affiliations
Arisa Ito, Keio Univ. (Japan)
Hiroki Matsuo, Keio Univ. (Japan)
Tsukasa Suenari, Keio Univ. (Japan)
Takuro Kajihara, Keio Univ. (Japan)
Takehiro Kimura, Keio Univ. School of Medicine (Japan)
Shunichiro Miyoshi, Keio Univ. School of Medicine (Japan)
Satoshi Ogawa, Keio Univ. School of Medicine (Japan)
Tsunenori Arai, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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