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Proceedings Paper

Real-time monitoring BimL interacting with Bcl-xL during UV-induced apoptosis
Author(s): Xianwang Wang; Shengnan Wu
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Paper Abstract

BimL is an important BH3-only protein, whose function is act upstream of apoptosis. However, the molecular role of BimL in apoptosis remains not well understood. Our previous reported shown that BimL was involved in the UV irradiation-induced apoptosis. Based on RNAi, FRET and co-immunoprecipitation techniques, in this study, in addition to well determine the role of BimL in UV-induced apoptosis, we particularly monitor the relationship between BimL and Bcl-xL during UV irradiation in both living and extracted cells. First, Knockdown of BimL by a small hairpin RNA confirmed this BH3-only member play an important role in the UV-induced apoptosis. Moreover, using FRET technique, we further revealed that BimL directly binds to Bcl-xL, and the combination of these complexes became more and more obviously during UV irradiation, which results were further highlighted by co-immunoprecipitation analysis. Thus, the data suggested that BimL directly binds Bcl-xL and play an important role during UV irradiation-induced apoptosis through suppresses the function of Bcl-xL.

Paper Details

Date Published: 6 March 2009
PDF: 9 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801Y (6 March 2009); doi: 10.1117/12.822629
Show Author Affiliations
Xianwang Wang, South China Normal Univ. (China)
Shengnan Wu, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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