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Proceedings Paper

The use of spider webs as passive bioaerosol collectors
Author(s): Daniel I. Mattei; Charles A. Bleckmann; David J. Bunker; Ike Maxis
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Paper Abstract

Spider webs were shown to be effective collectors of bioaerosols and airborne microorganisms. Spider webs were collected and analyzed for microbial content using two general microbial culture mediums. To be considered suitable passive collectors, webs had to satisfy three basic conditions; (1) collection of microorganisms without discrimination based on species or size, (2) collection under variable environmental conditions, and (3) saturation avoidance in the presence of strong microbial launching sources. Samples were collected from four locations near Wright-Patterson Air Force Base, OH, a waste water treatment facility, a commercial garden center, a secluded state park area, and a parking garage located within a medium size metropolitan area. These four locations provided appropriately varied environmental and physical conditions to test the collection parameters previously stated. A simple collection methodology was devised; microscopy cover glass slides were used as collection instruments. The methodology assured sterility during collection and permitted in situ microbial growth, observation, and enumeration. Microbial growth, both bacteria and fungi, were recovered from all collected spider web samples.

Paper Details

Date Published: 6 May 2009
PDF: 9 pages
Proc. SPIE 7324, Atmospheric Propagation VI, 73240W (6 May 2009); doi: 10.1117/12.822547
Show Author Affiliations
Daniel I. Mattei, Air Force Institute of Technology (United States)
Charles A. Bleckmann, Air Force Institute of Technology (United States)
David J. Bunker, Air Force Institute of Technology (United States)
Ike Maxis, National MASINT Management Office (United States)

Published in SPIE Proceedings Vol. 7324:
Atmospheric Propagation VI
Linda M. Wasiczko Thomas; G. Charmaine Gilbreath, Editor(s)

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