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Proceedings Paper

Ultra-high-speed bionanoscope for cell and microbe imaging
Author(s): T. Goji Etoh; Cuong Vo Le; Hiroyuki Kawano; Ikuko Ishikawa; Atshushi Miyawaki M.D.; Vu T. S. Dao; Hoang Dung Nguyen; Sayoko Yokoi; Shigeru Yoshida; Hitoshi Nakano; Kohsei Takehara; Yoshiharu Saito
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Paper Abstract

We are developing an ultra-high-sensitivity and ultra-high-speed imaging system for bioscience, mainly for imaging of microbes with visible light and cells with fluorescence emission. Scarcity of photons is the most serious problem in applications of high-speed imaging to the scientific field. To overcome the problem, the system integrates new technologies consisting of (1) an ultra-high-speed video camera with sub-ten-photon sensitivity with the frame rate of more than 1 mega frames per second, (2) a microscope with highly efficient use of light applicable to various unstained and fluorescence cell observations, and (3) very powerful long-pulse-strobe Xenon lights and lasers for microscopes. Various auxiliary technologies to support utilization of the system are also being developed. One example of them is an efficient video trigger system, which detects a weak signal of a sudden change in a frame under ultra-high-speed imaging by canceling high-frequency fluctuation of illumination light. This paper outlines the system with its preliminary evaluation results.

Paper Details

Date Published: 10 February 2009
PDF: 11 pages
Proc. SPIE 7126, 28th International Congress on High-Speed Imaging and Photonics, 712605 (10 February 2009); doi: 10.1117/12.822466
Show Author Affiliations
T. Goji Etoh, Kinki Univ. (Japan)
Cuong Vo Le, Kinki Univ. (Japan)
Hiroyuki Kawano, The Institute of Physical and Chemical Research RIKEN (Japan)
Ikuko Ishikawa, The Institute of Physical and Chemical Research RIKEN (Japan)
Atshushi Miyawaki M.D., The Institute of Physical and Chemical Research RIKEN (Japan)
Vu T. S. Dao, Kinki Univ. (Japan)
Hoang Dung Nguyen, Kinki Univ. (Japan)
Sayoko Yokoi, Kinki Univ. (Japan)
Shigeru Yoshida, Kinki Univ. (Japan)
Hitoshi Nakano, Kinki Univ. (Japan)
Kohsei Takehara, Kinki Univ. (Japan)
Yoshiharu Saito, Olympus Corp. (Japan)

Published in SPIE Proceedings Vol. 7126:
28th International Congress on High-Speed Imaging and Photonics
Harald Kleine; Martha Patricia Butron Guillen, Editor(s)

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