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Proceedings Paper

Digital pinhole filter used for detection of confocal microscopy images
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Paper Abstract

When conventional physical pinholes are used, the alignment of pinholes is always a problem. An iterative Wiener deconvolution filter is used to reject out-of-focus light, a sub-pixel centre location method is used to locate the centre of the pinhole, and the dimensions of the pinhole are optimized using the confocal microscopic theory. A filter with digital pinhole is thus developed and compared with filter with conventional physical pinhole through comparative tests. Experimental results indicate that the alignment of pinholes can be made easy, a filter with digital pinhole can be used to take place of a filter with conventional physical pinhole, and it can also reduce the pixel crosstalk resulting from multipoint illumination.

Paper Details

Date Published: 12 January 2009
PDF: 5 pages
Proc. SPIE 7133, Fifth International Symposium on Instrumentation Science and Technology, 71333N (12 January 2009); doi: 10.1117/12.821249
Show Author Affiliations
Xiangdong Huang, Harbin Institute of Technology (China)
Na Liu, Harbin Institute of Technology (China)
Junning Cui, Harbin Institute of Technology (China)

Published in SPIE Proceedings Vol. 7133:
Fifth International Symposium on Instrumentation Science and Technology
Jiubin Tan; Xianfang Wen, Editor(s)

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