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Proceedings Paper

Surface plasmon resonance imaging for parallelized detection of protein biomarkers
Author(s): Marek Piliarik; Lucie Párová; Hana Vaisocherová; Jiří Homola
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Paper Abstract

We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 × 10-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels.

Paper Details

Date Published: 18 May 2009
PDF: 8 pages
Proc. SPIE 7356, Optical Sensors 2009, 73560D (18 May 2009); doi: 10.1117/12.820882
Show Author Affiliations
Marek Piliarik, Institute of Photonics and Electronics (Czech Republic)
Lucie Párová, Institute of Photonics and Electronics (Czech Republic)
Hana Vaisocherová, Institute of Photonics and Electronics (Czech Republic)
Jiří Homola, Institute of Photonics and Electronics (Czech Republic)

Published in SPIE Proceedings Vol. 7356:
Optical Sensors 2009
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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