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Proceedings Paper

Laser rastering flow cytometry: fast cell counting and identification
Author(s): G. Vacca; M. R. Junnarkar; N. R. Goldblatt; M. W. Yee; B. M. Van Slyke; T. C. Briese
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Paper Abstract

We describe the concept of laser rastering flow cytometry, where a rapidly scanning laser beam allows counting and classification of cells at much higher rates than currently possible. Modifications to existing flow cytometers to implement the concept include an acousto-optic deflector, fast analog-to-digital conversion, and a two-step digital-signal-processing scheme that handles the high data rates and provides key assay information. Results are shown that prove the concept, demonstrating the ability to resolve closely spaced cells and to measure cells at rates more than an order of magnitude faster than on conventional flow-cytometer-based hematology analyzers.

Paper Details

Date Published: 12 February 2009
PDF: 10 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821T (12 February 2009); doi: 10.1117/12.820799
Show Author Affiliations
G. Vacca, Abbott Hematology (United States)
M. R. Junnarkar, Abbott Hematology (United States)
N. R. Goldblatt, Abbott Hematology (United States)
M. W. Yee, Abbott Hematology (United States)
B. M. Van Slyke, Plexus Technology Group (United States)
T. C. Briese, Plexus Technology Group (United States)

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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