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Proceedings Paper

Ex vivo and in vivo OCT image contrast
Author(s): N. Krstajić; J. Jacobs; M. Bonesi; L. E. Smith; P. Deshpande; S. MacNeil; R. Smallwood; S. J. Matcher
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Paper Abstract

We present results of OCT and polarization-OCT applied to various ex vivo tissue samples and discuss related issues of image contrast, comparing in vivo and ex vivo preparations. Time-domain and frequency-domain OCT at 835nm and 1300nm have been applied to ex vivo skin and rabbit cornea. We can distinguish rabbit cornea epithelium for up to a month after excision. However, the skin loses all contrast upon excision and despite numerous experiments we cannot distinguish epidermis, which is clearly visible in vivo. Using a time-domain system, birefringence is clearly visible for decalcified tissue but can also be detected more weakly on fully mineralised tissue. Analysis suggests that demineralization increases the birefringence value.

Paper Details

Date Published: 30 December 2008
PDF: 8 pages
Proc. SPIE 7139, 1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics, 71390W (30 December 2008); doi: 10.1117/12.819495
Show Author Affiliations
N. Krstajić, Univ. of Sheffield (United Kingdom)
J. Jacobs, Kroto Research Institute (United Kingdom)
M. Bonesi, Kroto Research Institute (United Kingdom)
L. E. Smith, Kroto Research Institute (United Kingdom)
P. Deshpande, Kroto Research Institute (United Kingdom)
S. MacNeil, Kroto Research Institute (United Kingdom)
R. Smallwood, Univ. of Sheffield (United Kingdom)
S. J. Matcher, Kroto Research Institute (United Kingdom)

Published in SPIE Proceedings Vol. 7139:
1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics
Adrian Podoleanu, Editor(s)

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