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Proceedings Paper

Lab-on-a-chip PCR in continuous flow: an ultrafast analytical tool for B-agents
Author(s): Claudia Gärtner; Holger Becker; Thomas Clemens; Richard Klemm; Matthias Klotz; Nadine Hlawatsch
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Paper Abstract

The overall aim is the realization of a reliable, ultrafast, and portable tool for the identification of B-agents at the point of interest. PCR is the method to be used for the doubtless identification of e.g. bacteria, and viruses. Miniaturization is the way to include the overall analysis process, from sample preparation to detection, on a microtiterplate-sized consumable device and to allow to carry out the analysis without the need for an equipped biological laboratory. The novel PCR concept with constant temperature zones allows also for the instrument to become portable, due to much reduced power consumption since no thermocycling is necessary. This paper focuses on the overall concept to implement the biological reactions for the analysis on-chip, the methods of sample preparation on-chip, and the results of the ultrafast PCR with B-agents on-chip, as well as the basic instrument.

Paper Details

Date Published: 8 May 2009
PDF: 6 pages
Proc. SPIE 7304, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X, 73040G (8 May 2009); doi: 10.1117/12.819429
Show Author Affiliations
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)
Holger Becker, microfluidic ChipShop GmbH (Germany)
Thomas Clemens, Clemens GmbH (Germany)
Richard Klemm, microfluidic ChipShop GmbH (Germany)
Matthias Klotz, Clemens GmbH (Germany)
Nadine Hlawatsch, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 7304:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X
Augustus W. Fountain III; Patrick J. Gardner, Editor(s)

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