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Proceedings Paper

Phage-based magnetoelastic biosensor for the detection of Salmonella typhimurium
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Paper Abstract

In this paper, we report a wireless magnetoelastic (ME) biosensor with phage as the bio-recognition probe for real time detection of Salmonella typhimurium. The ME biosensor was constructed by immobilizing filamentous phage that specifically binds with S. typhimurium onto the surface of a strip-shaped ME particle. The ME sensor oscillates with a characteristic resonance frequency when subjected to a time varying magnetic field. Binding between the phage and antigen (bacteria) causes a shift in the sensor's resonance frequency. Sensors with different dimensions were exposed to various known concentrations of S. typhimurium ranging from 5 x101 to 5 x 108 cfu/ml. The detection limit of the ME sensors was found to improve as the size of the sensor became smaller. The detection limit was found to improve from 161 Hz/decade (2mm length sensors) to 1150 Hz/decade (500 μm length sensors). The stability of the ME biosensor was investigated by storing the sensor at different temperatures (25, 45, and 65 °C), and then evaluating the binding activity of the stored biosensor after exposure to S. typhimurium solution (5 x 108 cfu/ml). The results showed that the phage-coated biosensor is robust. Even after storage in excess of 60 days at 65 °C, the phage-coated sensors have a greater binding affinity than the best antibody coated sensors stored for 1 day at 45 °C. The antibody coated sensors showed near zero binding affinity after 3 days of storage at 65 °C.

Paper Details

Date Published: 27 April 2009
PDF: 10 pages
Proc. SPIE 7315, Sensing for Agriculture and Food Quality and Safety, 731503 (27 April 2009); doi: 10.1117/12.818315
Show Author Affiliations
Suiqiong Li, Auburn Univ. (United States)
Ramji S. Lakshmanan, Auburn Univ. (United States)
Rajesh Guntupalli, Auburn Univ. (United States)
Shichu Huang, Auburn Univ. (United States)
Z.-Y. Cheng, Auburn Univ. (United States)
Valery A. Petrenko, Auburn Univ. (United States)
James M. Barbaree, Auburn Univ. (United States)
Vitaly Vodyanoy, Auburn Univ. (United States)
Bryan A. Chin, Auburn Univ. (United States)

Published in SPIE Proceedings Vol. 7315:
Sensing for Agriculture and Food Quality and Safety
Moon S. Kim; Shu-I Tu; Kaunglin Chao, Editor(s)

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