We have demonstrated the use of the Opto-Fluidic ring resonator (OFRR) to achieve the label-free detection of CD4+ and CD8+ T-Lymphocytes. The OFRR sensing technology combines microfluidics and optical sensing in a small platform that achieves rapid detection. In this work, white blood cells were obtained from healthy blood and the concentration altered to reflect CD4 and CD8 concentrations of HIV infected individuals. The OFRR was modified to effectively capture these receptors located on T-Lymphocytes and obtain a sensing signal through interaction with an evanescent field. Results show isolation of CD4+ and CD8+ T-Lymphocytes at medically significant levels. This work will lead to a device that can provide a CD4 and CD8 count to measure HIV progression in a low cost sensing setup.

Date Published: 29 April 2009
PDF: 7 pages
Proc. SPIE 7322, Photonic Microdevices/Microstructures for Sensing, 73220D (29 April 2009); doi: 10.1117/12.818255

Published in SPIE Proceedings Vol. 7322:
Photonic Microdevices/Microstructures for Sensing
Hai Xiao; Xudong Fan; Anbo Wang, Editor(s)