Share Email Print

Proceedings Paper

Near infrared probes for biochemical, cellular, and whole animal analysis of disease processes
Author(s): Joy Kovar; Vince Boveia; Huaxian Chen; Xinzhan Peng; Rose Skopp; Garrick Little; Dan Draney; D. M. Olive
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The study of disease processes requires a number of tools for detection of proteins and biomarkers in cell and animal based assays. Near infrared (NIR) technologies offer the advantage of high signal without interference from background producing factors such as tissues, blood, or plastics. NIR fluorescence quenching biochemical assays employing a novel NIR quencher are homogeneous and sensitive. NIR-based immunocytochemical assays offer a means of quantitatively evaluating cell signaling pathways. The technology can be extended to the development of targeted molecular imaging agents for disease analysis in animal models. We describe here model assays for each of these categories. A fluorescence quenching caspase-3 assay was developed employing a novel, broadly applicable quencher dye suitable for use with both visible and NIR dye chemistries. An NIR cell based assay is described for assessment of phosphorylation of p53 in response to a cellular stimulus. Finally, we describe the development and application of a targeted NIR optical imaging agent for monitoring tumor growth in whole animals. The NIR biochemical and cell based assays are robust with Z' factors greater than 0.7. The use of an IRDye (R)800CW-labeled cyclic RGD peptide is presented as a model for development and application of targeted imaging agents. NIR technologies are compatible with the complete spectrum of assay needs for disease analysis and therapeutic development.

Paper Details

Date Published: 20 February 2009
PDF: 8 pages
Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 71900N (20 February 2009); doi: 10.1117/12.815542
Show Author Affiliations
Joy Kovar, LI-COR Biosciences (United States)
Vince Boveia, LI-COR Biosciences (United States)
Huaxian Chen, LI-COR Biosciences (United States)
Xinzhan Peng, LI-COR Biosciences (United States)
Rose Skopp, LI-COR Biosciences (United States)
Garrick Little, LI-COR Biosciences (United States)
Dan Draney, LI-COR Biosciences (United States)
D. M. Olive, LI-COR Biosciences (United States)

Published in SPIE Proceedings Vol. 7190:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?