Proceedings Paper
Transient state microscopy: a new tool for biomolecular imaging| Format | Member Price | Non-Member Price |
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Paper Abstract
Photoinduced transient dark states are exhibited by practically all common fluorophores. However, their information
content has to date only been sparsely exploited due to methodological constraints. Here, a new concept is presented and
verified that can monitor and image these states via their photodynamic fingerprints. It unites the environmental
sensitivity of these states with the sensitivity of
fluorescence-based detection. For demonstration, triplet state images of
liposomes in different environments were generated, showing how local environmental differences can be resolved, not
clearly distinguishable via other fluorescence parameters. The concept can provide several new, useful and independent
fluorescence-based parameters in biomolecular imaging.
Paper Details
Date Published: 13 February 2009
PDF: 11 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832R (13 February 2009); doi: 10.1117/12.814908
Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)
PDF: 11 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832R (13 February 2009); doi: 10.1117/12.814908
Show Author Affiliations
Tor Sandén, Royal Institute of Technology (Sweden)
Gustav Persson, Royal Institute of Technology (Sweden)
Gustav Persson, Royal Institute of Technology (Sweden)
Jerker Widengren, Royal Institute of Technology (Sweden)
Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)
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