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Proceedings Paper

Transient state microscopy: a new tool for biomolecular imaging
Author(s): Tor Sandén; Gustav Persson; Jerker Widengren
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Paper Abstract

Photoinduced transient dark states are exhibited by practically all common fluorophores. However, their information content has to date only been sparsely exploited due to methodological constraints. Here, a new concept is presented and verified that can monitor and image these states via their photodynamic fingerprints. It unites the environmental sensitivity of these states with the sensitivity of fluorescence-based detection. For demonstration, triplet state images of liposomes in different environments were generated, showing how local environmental differences can be resolved, not clearly distinguishable via other fluorescence parameters. The concept can provide several new, useful and independent fluorescence-based parameters in biomolecular imaging.

Paper Details

Date Published: 13 February 2009
PDF: 11 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832R (13 February 2009); doi: 10.1117/12.814908
Show Author Affiliations
Tor Sandén, Royal Institute of Technology (Sweden)
Gustav Persson, Royal Institute of Technology (Sweden)
Jerker Widengren, Royal Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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