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Proceedings Paper

Near infrared fluorescence imaging of small animals with simultaneously estimated structural information
Author(s): Nrusingh C. Biswal; John Gamelin; Baohong Yuan; Joseph M. Backer; Quing Zhu
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Paper Abstract

We present a robust technique for diffuse optical fluorescence imaging of tumors in mice and tissue simulating fluorescence phantoms. The detection optics, which is a crucial part of a frequency domain fluorescence imaging system, with appropriate optical filters for efficient rejection of the excitation light, is demonstrated. The image reconstruction is divided into two parts; i.e. reconstructing the target locations such as size and position, and reconstructing the functional information such as fluorophore concentration and image reconstruction. The structural parameters i.e. tumor size and locations of the targets are recovered by a chi-square fitting technique by fitting the experimental data into analytically generated data. Having the structural information beforehand, the images are reconstructed by using our dual-mesh technique. The fluorescence images of targets of few tens of nanomolar fluorophore concentrations in both homogeneous and heterogeneous media are reconstructed in this study.

Paper Details

Date Published: 23 February 2009
PDF: 10 pages
Proc. SPIE 7174, Optical Tomography and Spectroscopy of Tissue VIII, 717416 (23 February 2009); doi: 10.1117/12.812083
Show Author Affiliations
Nrusingh C. Biswal, Univ. of Connecticut (United States)
John Gamelin, Univ. of Connecticut (United States)
Baohong Yuan, The Catholic Univ. of America (United States)
Joseph M. Backer, SibTech, Inc. (United States)
Quing Zhu, Univ. of Connecticut (United States)

Published in SPIE Proceedings Vol. 7174:
Optical Tomography and Spectroscopy of Tissue VIII
Bruce J. Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva M. Sevick-Muraca; Robert R. Alfano, Editor(s)

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