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Proceedings Paper

Developing new fluorescent proteins with stagger extension process
Author(s): Jie Yang; Jinling Lu; Haiming Luo; Qingming Luo; Zhihong Zhang
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Paper Abstract

The Stagger Extension Process (StEP), a recombination of DNA technique, has been used as a rapid molecular mutagenesis strategy. In this study, for obtaining the fluorescence proteins with new properties, six fluorescence proteins (EYFP, EGFP, ECFP, mCitrine, mCerulean and Venus) were used as the templates to recombine the mutation library by the Stagger Extension Process (StEP) technique. Through screening this mutation library, we have obtained some useful new FPs which are different fluorescent properties with ancestor. These protein will extend fluorescent proteins application.

Paper Details

Date Published: 16 February 2009
PDF: 5 pages
Proc. SPIE 7191, Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV, 71910H (16 February 2009);
Show Author Affiliations
Jie Yang, Huazhong Univ. of Science and Technology (China)
Jinling Lu, Huazhong Univ. of Science and Technology (China)
Haiming Luo, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 7191:
Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV
Alexander P. Savitsky; Yingxiao Wang, Editor(s)

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