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Proceedings Paper

Low-cost, multiplexed biosensor for disease diagnosis
Author(s): Christopher J. Myatt; Marie Delaney; Kathryn Todorof; James Heil; Monique Givens; Robert T. Schooley M.D.; Michael J. Lochhead
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Paper Abstract

Cost-effective disease diagnosis in resource-limited settings remains a critical global health challenge. Qualitative rapid tests based on lateral flow technology provide valuable screening information, but require relatively expensive confirmatory tests and generally lack quantitation. We report on a fluorescence technology that combines low cost instrumented readout with passive pumping in a disposable cartridge. The detection system utilizes a novel waveguide illumination approach in conjunction with commercial CMOS imagers. Total instrument cost in production are projected to be around $100 This cost structure and instrument ease of use will enable use in point-of-care settings, outside of centralized laboratories. The system has been used for detection and analysis of proteins, antibodies, nucleic acids, and cells. Here we will report first on our development of a multiplexed, array-based serology assay for HIV and common AIDS co-infections. Data will be presented for HIV/HCV antibody testing in human serum samples. In addition, we will present data on the use of the system for sensitive detection of bacterial RNA. Current detection limit for the model multiplexed RNA sandwich assay is 1 femtomolar target RNA. Finally, a high magnification version of the system is used to image immunostained human T cells.

Paper Details

Date Published: 19 February 2009
PDF: 9 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 716703 (19 February 2009); doi: 10.1117/12.811129
Show Author Affiliations
Christopher J. Myatt, Precision Photonics Corp. (United States)
Marie Delaney, Precision Photonics Corp. (United States)
Kathryn Todorof, Precision Photonics Corp. (United States)
James Heil, Precision Photonics Corp. (United States)
Monique Givens, Precision Photonics Corp. (United States)
Robert T. Schooley M.D., Univ. of California, San Diego (United States)
Michael J. Lochhead, Precision Photonics Corp. (United States)

Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

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