Laser scanning systems for two-photon microscopy and fabrication have been proven to be excellent in depth-resolving capability for years. However, their applications have been limited to laboratory use due to their intrinsic slow nature. The recently introduced temporal focusing concept enables wide-field optical sectioning and thus has potential in both high-speed 3D imaging and 3D mass-production fields. In this paper, we use the ultrafast optical pulse manipulation to generate two-photon excitation depth-resolved wide-field illumination (TPEDRWFI). The design parameters for the illumination were chosen based on numerical simulation of the temporal focusing. The imaging system was implemented, and the optical sectioning performance was compared with experimental result.

Date Published: 13 February 2009
PDF: 8 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831B (13 February 2009); doi: 10.1117/12.810910

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)