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Proceedings Paper

Study on microvisualizing assay of delivered drug infiltration using 2-color optical coherence dosigraphy
Author(s): Yu Nakamichi; Souichi Saeki; Takashi Saito; Takafumi Hiro; Masunori Matsuzaki M.D.
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Paper Abstract

Recently, clinical treatments applying drug delivery system (DDS) have been being developed. However, it is quite difficult to in vivo diagnose spatiotemporal distribution of drug infiltration, so the validation study should be too insufficient to progress the DDS development. In this study, we propose a visualizing assay of DDS, namely 2-Color Optical Coherence Dosigraphy (2C-OCD). 2C-OCD is based on optical coherence tomography using two waveband "2-Color" light sources having different optical absorbance of drug. This can simultaneously provide microscale tomographic images of scatterer density and drug concentration. In order to evaluate the efficacy of this technique, this was applied to drug-diffusion phenomena in microchannel and lipidrich plaques of rabbit with drug administration, respectively. As a result of diffusion experiment, it was confirmed that 2C-OCD can visualize a cross-sectional map of drug concentration, with spatial resolution 5 micro m × 10 μm and accuracy plus-minus 13.0 μM. In ex vivo animal experiment, the enhancement of absorptivity could be observed inside lipidrich plaques, in which DDS drug could be therein uptaken by drug administration. The absorption maps corresponding to drug concentration were calculated, comparing with their histological images. Consequently, they had good coincidence with histological examinations, therefore, it was concluded that 2C-OCD could visualize drug infiltration in biological tissue with almost the same spatial resolution as OCT system.

Paper Details

Date Published: 23 February 2009
PDF: 9 pages
Proc. SPIE 7176, Dynamics and Fluctuations in Biomedical Photonics VI, 71760E (23 February 2009); doi: 10.1117/12.810775
Show Author Affiliations
Yu Nakamichi, Yamaguchi Univ. (Japan)
Souichi Saeki, Yamaguchi Univ. (Japan)
Takashi Saito, Yamaguchi Univ. (Japan)
Takafumi Hiro, Yamaguchi Univ. (Japan)
Masunori Matsuzaki M.D., Yamaguchi Univ. (Japan)

Published in SPIE Proceedings Vol. 7176:
Dynamics and Fluctuations in Biomedical Photonics VI
Valery Viktorovich Tuchin; Lihong V. Wang; Donald D. Duncan, Editor(s)

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