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Proceedings Paper

Microfluidic chip based hematoanalyzer using polyelectrolytic gel electrodes
Author(s): Kwang Bok Kim; Honggu Chun; Hee Chan Kim; Taek Dong Chung
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Paper Abstract

We reports on a novel microfluidic chip with polyelectrolytic gel electrodes (PGEs) used to rapidly count the number of red blood cells in diluted whole blood. The number and amplitude of dc impedance peaks provide the information about the number and size of red blood cells, respectively. This system features a low-voltage dc detection method and noncontact condition between cells and metal electrodes. The performance of this PGEs-based system was evaluated in three steps. First, in order to observe the size-only dependence of the impedance signal, three different sizes of fluorescent microbeads were used in the experiment. Second, the cell counting performance was evaluated by using 7.2 μm fluorescent microbeads, similar in size to red blood cells, in various concentrations and comparing the results with an animal hematoanalyzer. Finally, in human blood sample tests, intravenously collected whole blood was just diluted in a phosphate buffered saline without centrifuge or other pretreatments. The PGEs-based system produced almost identical numbers of red blood cells in over 800-fold diluted samples to the results from a commercialized human hematoanalyzer.

Paper Details

Date Published: 23 February 2009
PDF: 8 pages
Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070S (23 February 2009); doi: 10.1117/12.810648
Show Author Affiliations
Kwang Bok Kim, Seoul National Univ. (Korea, Republic of)
Honggu Chun, The Univ. of North Carolina at Chapel Hill (United States)
Hee Chan Kim, Seoul National Univ. (Korea, Republic of)
Taek Dong Chung, Seoul National Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 7207:
Microfluidics, BioMEMS, and Medical Microsystems VII
Wanjun Wang, Editor(s)

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