We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.

Date Published: 12 February 2009
PDF: 8 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821D (12 February 2009); doi: 10.1117/12.810548

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)