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Proceedings Paper

A container for the advanced cytometry standard (ACS)
Author(s): Robert C. Leif; Josef Spidlen; Ryan R Brinkman
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Paper Abstract

Introduction: The highest priority for the Advanced Cytometry Standard (ACS) is the interpretation of list-mode cytometry measurements. Other priorities of lesser importance are the capacity to reproduce a cytometry measurement and the implementation of a digital microscopy image standard. The sequential nature of these requirements is being accommodated by a flexible, modular design. A major feature of this modular design is the creation of a design for an Advanced Cytometry Standard Container (ACSC) that includes a Table of Contents (ToC) XML file, one or more binary data containing files and files that contain the meta-data that describes the binary data. Methods: The design and partial implementation of the CytometryML schemas have been based on the techniques of modularity (each schema describing one object), iterative (spiral) development, inheritance, and reuse. Data-types including their definitions have been reused from DICOM, FCS, and other standards. Results: A prototype ToC schema together with prototypes of many of the schemas that describe the contents of the ACSC have been created together with their supporting schemas. These schemas have been validated with two tools and XML pages were generated from the main element(s) of the highest level schemas. These elements describe the table of contents of the zipped container file and a flow-cytometry instrument. The zipped container file (ACSC) describes and contains the meta and binary data.

Paper Details

Date Published: 12 February 2009
PDF: 11 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821Q (12 February 2009); doi: 10.1117/12.810195
Show Author Affiliations
Robert C. Leif, Newport Instruments (United States)
Josef Spidlen, BC Cancer Research Ctr. (Canada)
Ryan R Brinkman, BC Cancer Research Ctr. (Canada)

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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