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Proceedings Paper

Functionalised nanoparticles and SERRS for bioanalysis
Author(s): Duncan Graham; David G. Thompson; Fiona Mckenzie; Karen Faulds; Ross Stevenson; Andrew Ingram; Robert Stokes; Emma McFarlane; James Alexander; Paul Garside; Axel Huber; Ian McInnes
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Paper Abstract

Functionalised nanoparticles have been used in a number of studies including detection of DNA at ultra low concentrations, immuno-histochemistry and more recently as substrates for surface enhanced resonance Raman scattering (SERRS) based imaging approaches. The advantages of using metallic nanoparticles are that they are very bright in terms of their optical characteristics and also can be functionalised to provide a SERRS response and hence provide a unique Raman fingerprint. Here we present the functionalisation of gold and silver nanoparticles in such a way that the enhancement effect can be greatly increased through biological interaction and as such effectively turn on the SERRS effect. In an advancement of this nanoparticles have been used as imaging agents for single cells when functionalised with an appropriate antibody and can give information on the expression of specific receptors on cell surfaces as well as sub-cellular compartmentalisation information.

Paper Details

Date Published: 13 February 2009
PDF: 14 pages
Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 719202 (13 February 2009); doi: 10.1117/12.810161
Show Author Affiliations
Duncan Graham, Univ. of Strathclyde (United Kingdom)
David G. Thompson, Univ. of Strathclyde (United Kingdom)
Fiona Mckenzie, Univ. of Strathclyde (United Kingdom)
Karen Faulds, Univ. of Strathclyde (United Kingdom)
Ross Stevenson, Univ. of Strathclyde (United Kingdom)
Andrew Ingram, Univ. of Strathclyde (United Kingdom)
Robert Stokes, Univ. of Strathclyde (United Kingdom)
Emma McFarlane, Univ. of Strathclyde (United Kingdom)
James Alexander, Univ. of Strathclyde (United Kingdom)
Paul Garside, Univ. of Strathclyde (United Kingdom)
Axel Huber, Univ. of Glasgow (United Kingdom)
Ian McInnes, Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 7192:
Plasmonics in Biology and Medicine VI
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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