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Proceedings Paper

Stimulated Raman scattering microscopy for biomedical imaging
Author(s): Wei Min; Christian W. Freudiger; Sijia Lu; Chengwei He; Jing X. Kang; X. Sunney Xie
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Paper Abstract

Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a 3D multi-photon vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS is significantly greater than that of spontaneous Raman scattering, and is further enhanced by high-frequency (MHz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and easily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast.

Paper Details

Date Published: 13 February 2009
PDF: 7 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718320 (13 February 2009); doi: 10.1117/12.809802
Show Author Affiliations
Wei Min, Harvard Univ. (United States)
Christian W. Freudiger, Harvard Univ. (United States)
Sijia Lu, Harvard Univ. (United States)
Chengwei He, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Jing X. Kang, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
X. Sunney Xie, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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