Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

Date Published: 12 February 2009
PDF: 7 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718210 (12 February 2009); doi: 10.1117/12.809761

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)