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Proceedings Paper

Patterning of fibronectin using laser writer for force measurement in cells
Author(s): B. Codan; T. Gaiotto; R. Di Niro; R. Marzari; V. Sergo
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Paper Abstract

Mechanical forces and living cells are closely related, in particular in connections, named Focal Adhesions (FAs), between cells and extracellular matrix. FAs are mechanosensors and regulate physiological processes. The aim of this contribution is to explore the possibility of performing micrometric and submicrometric protein pattern in order to study FAs on different materials. Typical substrates for microelectromechanical systems (MEMS) are tested and the results are reported. A laser is used to produce a pattern of extracellular matrix proteins, like fibronectin. A new use of a Raman microprobe is described. Cells arrange in a regular shape, following the geometry of the pattern. Protein spots last for more than 24 hours. It is very important to have a complete control on FAs and the technique proposed is a suitable solution.

Paper Details

Date Published: 16 February 2009
PDF: 8 pages
Proc. SPIE 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI, 71880P (16 February 2009); doi: 10.1117/12.809698
Show Author Affiliations
B. Codan, Univ. degli Studi di Trieste (Italy)
T. Gaiotto, Univ. degli Studi di Trieste (Italy)
R. Di Niro, Univ. of Oslo (Norway)
R. Marzari, Univ. degli Studi di Trieste (Italy)
V. Sergo, Univ. degli Studi di Trieste (Italy)

Published in SPIE Proceedings Vol. 7188:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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