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Proceedings Paper

Magnetic field effects on spectrally resolved lifetime of on-line oxygen monitoring using magneto-optic probes
Author(s): O. Mermut; P. Gallant; N. Le Bouch; S. Leclair; I. Noiseux; M. Vernon; J.-F. Morin; K. Diamond; M. S. Patterson; K. Samkoe; B. Pogue
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Paper Abstract

Multimodal agents that serve as both probes for contrast and light-activated effectors of cellular processes in diseased tissue were developed. These agents were introduced into multicellular tumor spheroids (3D tissue models) and in the chorioallantoic membrane (CAM) of a chicken embryo. The luminescence decay was examined using a novel technique involving a spectrally-resolved fluorescence lifetime apparatus integrated with a weak electromagnet. A spectrallyresolved lifetime setup was used to identify magneto-optic species sensitive to magnetic field effects and distinguish from background emissions. We demonstrate that the applied magnetic fields can alter reaction rates and product distribution of some dyes detected by time- and spectrally-resolved luminescence changes. We will discuss the use of exogenous magneto-optical probes taken up in tumors to both induce phototoxicity, a process that is governed by complex and dynamically evolving mechanisms involving reactive oxygen species, and monitor treatment progress. The magnetic field enhancement, measured over a range of weak fields (0-300 mT) is correlated to oxygenation and may be used to monitor dynamic changes occurring due to oxygen consumption over the course of photodynamic therapy. Such online measurements provide the possibility to derive real-time information about response to treatment via monitoring magnetic field enhancement/suppression of the time-resolved, spectrally-resolved luminescence of the probe at the site of the treatment directly. Magnetic perturbation of lifetime can serve as a status reporter, providing optical feedback of oxygen-mediated treatments in situ and allowing for real-time adjustment of a phototherapy treatment plan.

Paper Details

Date Published: 20 February 2009
PDF: 9 pages
Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 71900Y (20 February 2009); doi: 10.1117/12.809668
Show Author Affiliations
O. Mermut, National Optics Institute (Canada)
P. Gallant, National Optics Institute (Canada)
N. Le Bouch, National Optics Institute (Canada)
S. Leclair, National Optics Institute (Canada)
I. Noiseux, National Optics Institute (Canada)
M. Vernon, National Optics Institute (Canada)
J.-F. Morin, Univ. Laval (Canada)
K. Diamond, McMaster Univ. (Canada)
M. S. Patterson, McMaster Univ. (Canada)
K. Samkoe, Thayer School of Engineering (United States)
B. Pogue, Thayer School of Engineering (United States)

Published in SPIE Proceedings Vol. 7190:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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