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Proceedings Paper

Enhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents
Author(s): Adam de la Zerda; Zhuang Liu; Cristina Zavaleta; Sunil Bodapati; Robert Teed; Srikant Vaithilingam; Te-Jen Ma; Omer Oralkan; Xiaoyuan Chen; Butrus T. Khuri-Yakub; Hongjie Dai; Sanjiv S. Gambhir
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Paper Abstract

Photoacoustic imaging of living subjects offers high spatial resolution at increased tissue depths compared to purely optical imaging techniques. We have recently shown that intravenously injected single walled carbon nanotubes (SWNTs) can be used as targeted photoacoustic imaging agents in living mice using RGD peptides to target αvβ3 integrins. We have now developed a new targeted photoacoustic imaging agent based on SWNTs and Indocyanine Green (SWNT-ICG) with absorption peak at 780nm. The photoacoustic signal of the new imaging agent is enhanced by ~20 times as compared to plain SWNTs. The particles are synthesized from SWNT-RGD that noncovalently attach to multiple ICG molecules through pi-pi stacking interactions. Negative control particles had RAD peptide instead of RGD. We measured the serum stability of the particles and verified that the RGD/RAD conjugation did not alter the particle's absorbance spectrum. Finally, through cell uptake studies with U87MG cells we verified that the particles bind selectively to αvβ3 integrin. In conclusion, the extremely high absorption of the SWNT-ICG particles shows great promise for high sensitivity photoacoustic imaging of molecular targets in-vivo. This work lays the foundations for future in-vivo studies that will use the SWNT-ICG particles as imaging agents administered systemically.

Paper Details

Date Published: 24 February 2009
PDF: 8 pages
Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71772K (24 February 2009); doi: 10.1117/12.809601
Show Author Affiliations
Adam de la Zerda, Stanford Univ. (United States)
Zhuang Liu, Stanford Univ. (United States)
Cristina Zavaleta, Stanford Univ. (United States)
Sunil Bodapati, Stanford Univ. (United States)
Robert Teed, Stanford Univ. (United States)
Srikant Vaithilingam, Stanford Univ. (United States)
Te-Jen Ma, Stanford Univ. (United States)
Omer Oralkan, Stanford Univ. (United States)
Xiaoyuan Chen, Stanford Univ. (United States)
Butrus T. Khuri-Yakub, Stanford Univ. (United States)
Hongjie Dai, Stanford Univ. (United States)
Sanjiv S. Gambhir, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 7177:
Photons Plus Ultrasound: Imaging and Sensing 2009
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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